Immobilized Trypsin Finds Application in Bottom-Up Proteomics and Protein Sequencing, Mapping and Structural Studies
Immobilized Trypsin aids in digesting a range of concentrations of purified protein or complex protein mixtures. It has a wide range of applications that include protein sequencing, mapping and structural studies along with amino acid analysis. It produces very fast protein digestion and is highly selective in its cleavage of peptide bonds, which increases its importance in sequencing studies. Immobilized Trypsin finds application in bottom-up proteomics, which is widely used for characterization of complex protein samples. The process involves digestion of proteins to peptides with enzymes, typically trypsin. High-performance liquid chromatography is later used for peptide separation, followed by analysis with tandem mass spectrometry. Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. The trypsin offers efficient protein digestion within minutes. It lets you perform digestions in as little as 3...